Targeting P2X 7 receptor inhibits the metastasis of murine P388D1 lymphoid neoplasm cells to lymph nodes

Abstract

The P2X 7 R (P2X 7 receptor) is an ATP‐gated cation channel expressed in normal cells that participates in both cell proliferation and apoptosis. Here, we have confirmed P2X 7 R expression on murine P388D1 lymphoid neoplasm cells. In addition, ATP‐stimulated P2X 7 R expression was found to trigger increased intracellular calcium flux. Furthermore, silencing with short hairpin RNA and blocking with P2X 7 R antibody significantly reduced the metastasis of P388D1 cells to lymph nodes. These results indicate that inhibition of the expression and function of P2X 7 R attenuates the metastatic ability of murine lymphoid neoplasm cell line P388D1, which represents a new potential target for anti‐metastatic therapy.