Self-nanoemulsifying drug delivery system (SNEDDS) prepared from Begonia medicinalis and Moringa oleifera ethanol extracts

Abstract

Purpose: To investigate the activity of self-nanoemulsifying drug delivery system (SNEDDS) containing B. medicinalis and M. oleifera ethanolic extracts. Method: The SNEDDS was prepared using propylene glycol (40 %), Tween 80 (50 %), and isopropyl myristate (10 %). Transmittance percentage, particle size, polydispersity index, and zeta potential were evaluated. In vitro immunostimulant activity was measured by assessing the macrophages' phagocytic capacity and proliferation of lymphocyte cells using MTT assay. In vivo immunostimulant activity was determined by measuring the number (%) of leukocytes, neutrophils, and lymphocytes in Wistar male rats over 14 days following administration of the SNEDDS after induction with Staphylococcus aureus. Results: The SNEDDS formula of combined extract of B. medicinalis and M. oleifera demonstrated polydispersity index (0.46 ± 0.00), particle size (24.4 ± 0.1 nm), zeta potential (-37.73 ± 0.32 mV) and percentage transmittance (86.81 ± 0.16 %). In vitro and in vivo tests revealed that SNEDDS significantly increased macrophage phagocytic capacity, number of leukocytes, neutrophils and lymphocytes over 14 days of treatment (p < 0.05) following induction with S. aureus. Conclusion: Self-nanoemulsifying drug delivery system of combined ethanol extract of B. medicinalis and M. oleifera demonstrates stable physicochemical properties, and increases macrophage phagocytic activity, leukocytes, neutrophils and lymphocyte count.