Single-gene analysis has been the standard analytic method since DNA sequencing was originally performed. In practice, it was too time-consuming and expensive to perform analysis on more than a few exons or genes at a time. The nature of high-throughput sequencing has altered these calculations, and large panels of potentially pathogenic genes or even whole exomes or genomes are sequenced routinely. However, the techniques and equipment useful for high-throughput sequencing can be applied to single-gene targets in specialized situations. This chapter discusses these applications in minimal residual disease detection, infectious diseases, and HLA typing, among others.
CITATION STYLE
Ho, H., & Gocke, C. D. (2018). Next-Generation Sequencing for Single-Gene Analysis. In Genomic Applications in Pathology: Second Edition 2019 (pp. 183–190). Springer International Publishing. https://doi.org/10.1007/978-3-319-96830-8_15
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