Development of A Practicable Method for Isolation and Identification of Dengue Viruses in Developping Countries

Abstract

We sought a practicable method for isolation and identification of dengue viruses in South-East Asia. We compared two mosquito cell lines, C6/36 and TRA-284-SFG, for virus isolation and two identification methods, immunofluorescent staining of infected cells with serotype-specific mouse monoclonal antibodies and a sandwich-type enzyme-linked immunosorbent assay with conventional mouse hyperimmune ascitic fluids. We found that the combination of TRA-284-SFG cells and ELISA is a useful and feasible method in developping countries. © 1987, National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee. All rights reserved.