Erratum: Bisphenol-A Impairs Insulin Action and Up-Regulates Inflammatory Pathways in Human Subcutaneous Adipocytes and 3T3-L1 Cells (PLoS ONE 8: 12 (e82099) DOI: 10.1371/journal.pone.0082099)

Abstract

After publication of this article [1], concerns were raised about similarities between the Akt results shown in lanes 2–5 of Figure 3C and the Akt results shown in Fig 7C. The authors provided underlying image data for all panels shown in Figs 3 and 7 (S1–S4 Files). The Fig 7C Akt panel appears to align with the raw image for the Figure 3C Akt experiment (lanes 3–6 of the Akt blot in S2 File) suggesting that an error was made in preparing Fig 7C. The authors provided raw images from experimental replicates in support of Fig 7C results (S4 and S5 Files), as well as an updated Fig 7 including the correct Akt data. S5 File includes additional raw images, including different exposures of the original blots and images in which lanes included in the published figures are marked for easy reference. The raw image data revealed that the pAkt and pERK blot images were spliced to remove data between lanes 4 and 5 on the published Figure 3C (see S2 and S5 Files). In regard to the control panels included in Figs 3 and 7, the authors clarified that the images included are representative examples from a full cohort of experimental results that included duplicates of each experiment. As such, the total protein, phosphoprotein, and β-actin controls shown in the Figures may not correspond to the same experiment and/or samples. For each experiment involving phospho-protein, control blots with β-actin and total protein were used. Each replicate experiment used independently prepared protein samples. The raw data underlying other results in this article are available from the authors. The authors apologize for the errors in the published article.