CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections

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Abstract

Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45intCD49b+FcεRI+ cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of αIIbβ3 integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis (Nb). Moreover, CD41 can be used as a reliable marker for basophils, circumventing technical difficulties associated with FcεRI for basophil identification in a Nb infection model. In vitro anti-IgE cross-linking and IL-3 basophil stimulation showed that CD41 upregulation positively correlates with augmented surface expression of CD200R and increased production of IL-4/IL-13, indicating that CD41 is a basophil activation marker. Furthermore, we found that infection with Plasmodium yoelii 17X (Py17x) induced a profound basophilia and using Mcpt8DTR reporter mice as a basophil-specific depletion model, we verified that CD41 can be used as a marker to track basophils in the steady state and during infection. During malarial infection, CD41 expression on basophils is negatively regulated by IFN-γ and positively correlates with increased basophil IL-4 production. In conclusion, we provide evidence that CD41 can be used as both an identification and activation marker for basophils during homeostasis and immune challenge. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Bakocevic, N., Claser, C., Yoshikawa, S., Jones, L. A., Chew, S., Goh, C. C., … Ng, L. G. (2014). CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections. European Journal of Immunology, 44(6), 1823–1834. https://doi.org/10.1002/eji.201344254

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