Identification of a set of KSRP target transcripts upregulated by PI3K-AKT signaling

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Abstract

Background: KSRP is a AU-rich element (ARE) binding protein that causes decay of select sets of transcripts in different cell types. We have recently described that phosphatidylinositol 3-kinase/AKT (PI3K-AKT) activation induces stabilization and accumulation of the labile β-catenin mRNA through an impairment of KSRP function. Results: Aim of this study was to identify additional KSRP targets whose stability and steady-state levels are enhanced by PI3K-AKT activation. First, through microarray analyses of the AU-rich transcriptome in pituitary αT3-1 cells, we identified 34 ARE-containing transcripts upregulated in cells expressing a constitutively active form of AKT1. In parallel, by an affinity chromatography-based technique followed by microarray analyses, 12 mRNAs target of KSRP, additional to β-catenin, were identified. Among them, seven mRNAs were upregulated in cells expressing activated AKT1. Both steady-state levels and stability of these new KSRP targets were consistently increased by either KSRP knock-down or PI3K-AKT activation. Conclusion: Our study identified a set of transcripts that are targets of KSRP and whose expression is increased by PI3K-AKT activation. These mRNAs encode RNA binding proteins, signaling molecules and a replication-independent histone. The increased expression of these gene products upon PI3K-AKT activation could play a role in the cellular events initiated by this signaling pathway. © 2007 Ruggiero et al; licensee BioMed Central Ltd.

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Ruggiero, T., Trabucchi, M., Ponassi, M., Corte, G., Chen, C. Y., al-Haj, L., … Gherzi, R. (2007). Identification of a set of KSRP target transcripts upregulated by PI3K-AKT signaling. BMC Molecular Biology, 8. https://doi.org/10.1186/1471-2199-8-28

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