Spectrophotometric determination of protein in alkaline solution

Abstract

THIS method for the spectrophotometric determination of protein in alkaline solution was developed in order to determine the protein of Chlorella cells. It was not possible to obtain Chlorella-cell protein, readily, in acid solution. It was possible to break most of the cells by grinding mechanically with silica sand but this method is tedious and time-consuming. The latter point is important when it is necessary to measure physiological changes which proceed at a rapid rate. © 1958 Nature Publishing Group.