Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6

  • Chung C
  • Mele A
  • Allen A
  • et al.
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Abstract

The full length of HIV/R7/E − /GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank. The full length of HIV/R7/E − /GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank.

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Chung, C.-H., Mele, A. R., Allen, A. G., Costello, R., Dampier, W., Nonnemacher, M. R., & Wigdahl, B. (2020). Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6. Microbiology Resource Announcements, 9(18). https://doi.org/10.1128/mra.00179-20

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