Levels of Circulating TIMP‐2 and MMP2‐TIMP2 Complex Are Decreased in Squamous Cervical Carcinoma

Abstract

Background . The role of matrix metalloproteinase‐2 and ‐9 (MMP‐2, MMP‐9) in matrix degradation and metastasis has been described in various tumors. Their action is inhibited by their natural tissue inhibitor molecules TIMP‐1 and ‐2. Methods . The study population consisted of 12 squamous cervical carcinoma patients and 27 healthy volunteer control patients. MMP‐9, MMP‐2‐TIMP‐2 complex, TIMP‐1, and TIMP‐2 were analyzed from serum samples using enzyme‐linked immunoassay (ELISA). Results . The mean levels of serum TIMP‐2 and of MMP‐2‐TIMP‐2 complex were higher in healthy controls compared to patients with a malignant tumor. Serum TIMP‐2 values decreased significantly from healthy controls (median 323 μ g/l, range 305–342 μ g/l) to malignant (median 136 μ g/l, range 120–151 μ g/l) squamous cervical carcinoma patients ( P < .000). Also, serum proMMP2‐TIMP2 complex values decreased from control patients to squamous cervical carcinoma patients ( P < .006). Conclusion . This paper shows that the levels of circulating TIMP‐2 and that of MMP‐2‐TIMP‐2 complex are lower in squamous cervical carcinoma patients than in healthy women.