Simultaneous determination of five benzimidazoles in feeds using high-performance capillary electrophoresis

Abstract

A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm × 75 μm id fused-silica uncoated capillary and injected by hydrodynamic injection (35 mbar × 8 s) at a wavelength detection of 295 nm. The drugs were completely resolved in 50 mmol/L citric acid buffer (pH 1.9) with 30 kV at a temperature of 25°C. Linear range was 1-80 μg/mL; limits of detection for the analytes were less than 2 mg/kg; the overall recoveries for benzimidazoles were >73%, with coefficients of variation below 10%.