Promotion of Seed Germination by Cyanide

Abstract

paragine formed by hydrolysis from #-cyanoalanine, is the second most effective of the 23 amino acids. Uptake of as- partate-4-'C is much lower than of cyanide. Radioactive tracer in K14CN shows uptake of about 1.5 of Amaranthus albus, Lactuca sativa, and Lepidium rirginicunt seeds. L-Cysteine hydrogen sulfide lyase, which catalyzes the reaction of HCN with L-cysteine to form ,-L cyanoaLanine, is active in the seeds. 8-L-Cyanoalanine is the most effective of the 23 a-amino acids tested for promoting germination of A. albus seeds. Aspartate, which is produced by enzymatic hydrolysis of Potassium cyanide at 3 ,uM to 10 mM promotes germination activity in ,B-cyanoalanine, asparagine, and aspartate. The acid- hydrolyzed protein extract gave high activity only in aspartate. Tests were negative for free cyanide in the seed. Respiration of the seed is inhibited more than 75% by KCN and by KN3 at 10 mM. Azide at greater than 1.0 mM inhibits the promotion of germination by cyanides. Neither 0.1 mM KCN nor KN3 inhibit 02 consumption, whereas lower concentrations promote germination. It is concluded that the high rate of utilization of cyanide in the reaction to form ,B-L-cyanoalanine and the subsequent incorporation into protein limit any inhibition of oxygen consumption. The promotion of seed germination is substrate.limited by asparagine-aspartate, which is required for protein synthesis.