Identification of superior cellulose microbes producer for bioethanol production

Abstract

The limited supply of fossil fuels in the world and increased in CO2 emission problem causing the government has urged improving the supply of a source of energy, through the use of a new source of energy and renewable. Lignocellulose is one of the organic component in many was available an agricultural waste as a source of microbes to produce welfare fuel. In process of renewable energy, hydrolysis on source lignocellulose using a lignocellulose enzyme will be imported. Bioethanol produce there are have some problems, among other lack of strains superior enzyme lignocellulose microbes producer. Lignocellulose that degrades an enzyme is cellulose much used in various industries. An enzyme can be produced of a group of bacteria, like mold and yeasts. This article is determine to identify superior lignocellulose microbes producing an enzyme. Sample of the study obtained from the specimen land, litter, decayed wood, sand, water crater in various districts in Indonesia. Produce an enzyme lignocellulose identification microbes to bioetanol begins with exploration, isolation and selection, further testing and selection. From 15 bacteria producing cellulose isolates, and selection isolate superior obtained was that B93 with the activity of enzyme reached 25,3 U/ml. Identification of superior cellulose microbes producer is Enterobacteria Sp. Based on this outcome was expected that B93 is a potential source of an cellulose enzyme producer. That can be used to producing welfare fuel (bioethanol). The next research can get reducing part cost of bioethanol production from lignocellulose is cost of cellulose and xillanase (commercial enzim), so it is very impact alternative to solve the global warming problem and ensure sustainable development of the economy leaf litter biomass from tree plantation sites can be collected and used as a promising feedstock for biofuel production to mitigate energy crisis.