In vitro shoot bud differentiation from leaf segments of Achras sapota

Abstract

Direct shoot bud differentiation was achieved in leaf segments of Achras sapota cv. Cricket Ball inoculated on Schenk and Hildebrandt's medium supplemented with 5.0 μM thidiazuron and 8.88 μM benzylaminopurine. Leaves from middle part of the shoots and segments obtained from middle portion of leaf showed highest potential to regenerate shoot buds. Histological examination of developing shoot buds showed their de novo regeneration with clear vascular connection with the mother tissues.