Antioxidant Properties and Antibacterial Activities of Leptadenia Hastata Leaves Extracts on Staphylococcus Aureus

Abstract

Leptadenia hastata extracts were evaluated for antibacterial activity and antioxidant properties. The leaf of the plant was extracted with n-hexane, dichloromethane, ethyl acetate, chloroform and methanol to give respective extracts. The Antibacterial activity against Staphylococcus aureus, was determined by disc diffusion method. Antioxidant activity was assayed by the DPPH radical scavenging activity mechanism. The results showed that of n-hexane, ethyl acetate, dichloromethane, chloroform and methanol extracts of Leptadenia hastata, methanol extract and chloroform extracts displayed more activity with 1.10 Â 0.10ab and 0.97 Â 0.06ab than others at 25-1000ppm/desk of the extracts tested. The most antioxidant activities against DPPH were displayed by the hexane and methanol extracts of Leptadenia hastata leaf, exhibiting IC50 (63.44I14g/mL) and IC50 (46.80I14g/mL) respectively, followed by chloroform, dichloromethane and lastly ethyl acetate crude extract. The present results showed potential of the medicinal plant used by traditional herbal medical practitioners as natural anti-microbial, antioxidant and potentially antiinflammatory agents. The results confirm that Leptadenia hastata leaves extracts can be used as a source of drugs to fight infections caused by susceptible bacteria.