Impacts of Different Reforestation Methods on Fungal Community and Nutrient Content in an Ex-Tea Plantation

Abstract

Long-term monocultures of tea and the excessive use of chemical fertilizer lead to the degradation of soil quality. Improving the soil quality of ex-tea plantations through vegetation restoration is an important task. However, the changes in soil nutrients, fungal communities, and the effects of microorganisms on soil nutrients after reforestation remain unclear. Therefore, in this study, we aimed to explore the effects of Pinus and Chinese fir on soil nutrients and fungal communities in ex-tea plantation areas that were subjected to the reforestation modes of pure forest and mixed forest by measuring soil chemical properties and ITS rRNA gene sequences. The results showed that (1) after reforestation, the relative normalized difference vegetation index (NDVI) of the Mixed forest, Mixed Pine and Mixed Fir areas increased (p < 0.05) compared to that of pure forest; (2) the soil organic carbon (SOC), total nitrogen (TN), and N:P ratios of the mixed forest increased by an average of 54%, 90%, and 299% (p < 0.05) compared to pure forest, whereas the total phosphorus (TP) and available potassium (AK) decreased by an average of 39% and 89% (p < 0.05); and (3) there was no significant difference in the diversity of the fungal communities of the pure and mixed forests, but the fungal phyla Mucoromycota, Glomeromycota, and Rozellomycota were significantly different in the pure and mixed forests. This differing microbial composition led to a significant increase (p < 0.05) in symbiotrophs (ecotomycorhizal, ericoid mycorhizal) in the mixed forest, which was negatively correlated with the soil TP and positively correlated with the TN and the N:P ratio. In addition, there was also a significant decrease (p < 0.05) in complex nutrient types (ectomycorrhizal-fungal parasite-plant saprotroph-wood saprotroph), which were negatively correlated with the SOC and TN, and arbuscular mycorrhizas, which were positively correlated with the TP. Our results show that the chemical properties of soils and the structure of the fungal communities changed significantly due to the reforestation of Chinese fir and Pinus, and the mixed forest mode of reforestation was more conducive to improving the soil quality; therefore, a mixed forest of Chinese fir and Pinus can be used to improve degraded soils in ex-tea planting areas.