Validación De Un Método Analítico Para Cuantificar Aluminio En Tejidos De Ratas Sprague Dawley Mediante Eaa-Flama Y Eaa-Horno De Grafito

Abstract

A method to quantify aluminum (Al) in tissues of Sprague Dawley rats by flame atomic absorption spectrophotometry (F-AAS) and graphite furnace (GF-AAS) was validated. The linearity was evaluated by the correlation coefficient (r) and the results were 1.000 and 0.9993 for F-AAS and GF-AAS, respectively. Accuracy was 100.62 and 97.75 %, while the precision coefficient of variation was of 0.61 and 1.30 % for F-AAS and GF-AAS, respectively. Precision was also evaluated under repeatability conditions; the result obtained indicates that the method is repeatable for the Al concentrations used and determined by F-AAS and GF-AAS. The results of limit of detection and limit of quantification of Al determined by F-AAS were 0.06 and 0.19 mg/L, and 0.05 to 0.18 Μg/L of Al determined by GF-AAS. Furthermore, the accuracy of the analytical method was evaluated with oyster tissue as certified reference material (NIST-1566b) digested in microwave system and containing a concentration of 197.2 ± 6.0 mg of Al/kg of tissue. The results for accuracy through the percent recovery rate were 100.60 and 100.51 % for F-AAS and GF-AAS, respectively. Finally, the applicability of the method was evaluated by fortifying rats’ tissues and controls. The results for accuracy by the recovery rate ranged between 99.32 and 109.00 %.