A thin liquid film thickness shear mode bulk acoustic wave sensor for determination of Proteus mirabilis

Abstract

A thickness shear mode bulk acoustic wave sensor coated with a thin liquid culture medium film was developed and applied to determine the concentration of Proteus mirabilis (P. mirabilis). Experiments demonstrated that there was a good linear relationship between the turning point time and the logarithm of the P. mirabilis concentration in the range 2.0 × 102-2.0 × 106 cells ml-1. The detection was fast and accurate because of the sharp turning point of the response due to the thin culture film on the sensor surface. Other problems concerning the experiments are discussed in detail.