Polymorphism of glutamate-cysteine ligase subunit catalytic (GCLC) gene in pulmonary tuberculosis patients

Abstract

Background and Objective: The biomarker of oxidative stress in pulmonary tuberculosis patients has not been found. Oxidative stress occurs due to the low level of antioxidants. Single nucleotide polymorphism of glutamate-cysteine ligase subunit catalytic (GCLC) gene namely -129C/T GCLC has been reported to have an association with a risk factor of oxidative stress' susceptibility. The Objective of this study was to determine the GCLC polymorphism in pulmonary tuberculosis (TB) patient. Materials and Methods: Blood samples of 225 pulmonary TB patients were taken from the central public health in Semarang city. The genetic test was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The isolation of DNA from 225 blood samples was performed using DNA extraction kit (Promega DNA purification kit) following the manufacturing procedure. The amplification of GCLC fragment was performed by a master mix from Thermo Scientific. Data was analyzed descriptively. Statistical analysis was performed by Chi-square test. Results: The results showed the existence of polymorphism-129C/T in the 5'-flanking region of GCLC genes. The frequency of C/C and C/T genotype were 63.6 and 36.4%, respectively. The C/T gene in the GCLC -129C region is a T gene promoter. There was a significant different between C/C and C/T frequencies with the value of significance of p = 0.000 (p<0.05). Conclusion: Therefore it was concluded that the frequency of C/T polymorphism genotype of GCLC gene in patients with pulmonary tuberculosis is 36.4%.