Ecuaciones Espectrofotométricas Tricromáticas Para Ia Determinación De Clorofila a, b y c Y Sus Feofitinas,

Abstract

Using the chlorophylls a, b and c specific absorbance coefficients of Parsons and Strickland (1963), and the chlorophyll to phaeophytin abosorbance ratios of Lorenzen (1967), six linear equations of absorbance as a function of pigment concentration were established. Three correspond to the absorbance maxima of these pigments in 90% acetone, and three correspond to the absorbances after acidification. Solving this equation system we obtained expressions for the concentration of the six pigments. After a first calculation, one can decide which pigments have concentrations significantly above zero (i.e.: greater than 0.1 mg/m3), and then recalculate them with an equation system that only considers them. This way "noice" is minimized. To do this, a computer program was made to consider a series of equation systems, from one with six variables to one with one variable. To avoid turbidity problems after acidification we used a solution with 90% acetone and dimethyl sulfoxide in a one to one volume ratio as suggested by Shoaf and Lium (1976).