Evaluating the Anticancer Effects of High-Dilution Preparations of Carcinogens such as HIV virus, Hepatitis C virus, Ethanol and Cancer Tissues in in-vitro models

Abstract

Cancer is one of the leading causes of mortality. The recent experiments with high-diluted preparations have shown anticancer effects in in-vitro and vivo models. The fundamental principle of homeopathy suggests that the substances capable of producing certain diseases may have a capacity to alter the same disease if used in the ultra-dilute-potentized form. This hypothesis led to certain carcinogens for examining their potential anticancer efficacy. Sulforhodamine B assay helps determine the cytotoxicity in cell-based studies in evaluating anticancer agents. The protocol involved preparation of homeopathy dilutions, incubation of cells with homeopathy dilutions, SRB binding, and absorbance measurement. Cells were treated with 30 potencies of HIV nosode, Hepatitis C nosode, Carcinosin, Cancer nosode, and Ethanol along with positive control (Adriamycin). The preparations were tested in HeLa, HepG2, A549, MCF 7, T 24, Jurkat, SCC 40, and HL-60 cell lines. The homeopathic preparations have shown the anticancer activity measured as percentage growth inhibition. All the homeopathic preparations studied exhibited anticancer activity on HeLa, HepG2, A 549, T 24, and HL-60 cells. Carcinosin showed anticancer activity on the SCC 40 cells. Hepatitis C nosode, Carcinosin, and Cancer nosode have demonstrated the anticancer activity on breast cancer cell line MCF-7. None of the preparations exhibited anticancer activity on Human Leukemia Cell Line. High-dilution, potentized preparations of certain carcinogens have demonstrated anticancer, cytotoxic effects in the cell-line model, supporting the rationale of the fundamental homeopathic principle the Law of Similars, opening windows to its broader applications in healthcare.