Systematic investigation of lycopene effects in LNCaP cells by use of novel large-scale proteomic analysis software

Abstract

Lycopene, the red pigment of tomatoes, is a carotenoid with potent antioxidant properties. Although lycopene may function as a prostate cancer chemoprevention agent, little is known about its effects at the cellular level. To define general changes induced by treatment of cells with lycopene, and to gain insights into the possible chemoprevention properties of lycopene, we investigated changes in protein expression after lycopene treatment in human LNCaP cells. The high throughput proteomics data were then visualized and analyzed by novel biological protein pathway modeling software. Differentially expressed proteins were identified, and the data were analyzed by protein pathway simulation software, without the need for specialized programming, by importing pathway models from a number of sources or by creating our own. One notable outcome was the identification of a group of upregulated proteins involved in detoxification of reactive oxygen species. This finding suggests that a possible mechanism of lycopene chemoprevention is the stimulation of detoxification enzymes associated with the antioxidant response element. Novel biological pathway modeling software enhances analysis of large proteomics data. When applied to the analysis of proteins differentially expressed in prostate cancer cells upon treatment with lycopene, the up-regulation of detoxification enzymes was identified. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.