Effects of covering behavior and exposure to a predatory crab Charybdis japonica on survival and HSP70 expression of juvenile sea urchins Strongylocentrotus intermedius

Abstract

Predation is a complex process among predator, prey and environment. Juvenile sea urchins are more susceptible to predators than adults, which affects community structure. Behavior is involved in anti-predator responses by changes in the expression of anti-predator responsive genes. Here, we investigated the effects of exposure to a predatory crab Charybdis japonica and covering behavior on survival and HSP70 expression of juvenile sea urchins Strongylocentrotus intermedius. C. japonica consumed large numbers of juvenile S. intermedius in 12 hours with a mortality of 34.17±11.43%. Covering behavior did not significantly reduce predation. Exposure to C. japonica did not significantly upregulate HSP70 expression of juvenile S. intermedius in 12 hours. Covering behavior showed no significant regulative effect on the gene expression of HSP70 of juvenile S. intermedius exposed to C. japonica for 12 hours. The results indicate that the anti-predator function of covering behavior is limited and that HSP70 expression does not appear to play an important role in the anti-predator process of S. intermedius. © 2014 Zhao et al.