Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.

  • Frey M
  • Reinecke J
  • Grant S
  • et al.
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Abstract

An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.

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Frey, M., Reinecke, J., Grant, S., Saedler, H., & Gierl, A. (1990). Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins. The EMBO Journal, 9(12), 4037–4044. https://doi.org/10.1002/j.1460-2075.1990.tb07625.x

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