Characterization of Staphylococcus aureus isolated from subclinical mastitis of Peranakan Ettawa goat in Pekanbaru

Abstract

Staphylococcus aureus (S. aureus) causing mastitis needs to be identified as the basis of treatment. This study aimed to characterize S. aureus isolated from Peranakan Ettawa (PE) goat's milk in Pekanbaru. A total of 50 milk samples were collected from the Farm in Pekanbaru. Based on the cultural and biochemical properties and the amplification of the 23S rRNA, coa, and nuc genes, eight isolates (53.33%) could be identified as& aureus. The characterization of S. aureus based on the fermenting of Mannitol Salt Agar media, positive for Voges-Proskauer, catalase, and coagulase tests. The abilities of S. aureus isolated from goats to form p-hemolysis on blood agar plates, agglutinate rabbit blood, compact formed colonies in the Soft Serum Agar (SSA) test indicates the pathogen of the isolates to host.