Midday depression in photosynthesis: Effect on sucrose-phosphate synthase and ribulose-1,5-bisphosphate carboxylase in leaves of Prosopis juliflora (Swartz) DC

Abstract

The midday depression in net photosynthetic rate (P(N)) and stomatal conductance (g(s)) in Prosopis juliflora was studied in relation to two key enzymes of carbon metabolism. Diurnal gas exchange measurements carried out in autumn on P. juliflora showed a pronounced depression in P(N) and g(s) along with a decrease in apparent carboxylation efficiency (CE*) during midday. The activities of sucrose-phosphate synthase (SPS) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) also showed large diurnal fluctuations. Initial RuBPCO activity (that present in vivo) and total activity (fully carbamylated activity) increased gradually with increase in irradiance, in the morning reaching a maximum by 08:00 h. The RuBPCO activity declined sharply during midday due to decrease in initial activity. The drop in the % activation of RuBPCO indicated that the deactivation of RuBPCO was achieved via a decarbamylation mechanism. There was a marked similarity in the diurnal patterns of SPS activity and the P(N). During a diurnal rhythm, SPS activity increased after irradiation, reaching a maximum at 08:00 h and then declined during midday. Diurnal fluctuations in SPS activity could be due to the changes in the amount of protein (changes in V(max)) as well as to the changes in kinetic properties (changes in V(lim)). Hence the midday decline in photosynthesis is closely associated with the regulation of RuBPCO and SPS activities.