Hi-D: Nanoscale mapping of nuclear dynamics in single living cells

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Bulk chromatin motion has not been analyzed at high resolution. We present Hi-D, a method to quantitatively map dynamics of chromatin and abundant nuclear proteins for every pixel simultaneously over the entire nucleus from fluorescence image series. Hi-D combines reconstruction of chromatin motion and classification of local diffusion processes by Bayesian inference. We show that DNA dynamics in the nuclear interior are spatially partitioned into 0.3-3-μm domains in a mosaic-like pattern, uncoupled from chromatin compaction. This pattern was remodeled in response to transcriptional activity. Hi-D can be applied to any dense and bulk structures opening new perspectives towards understanding motion of nuclear molecules.




Shaban, H. A., Barth, R., Recoules, L., & Bystricky, K. (2020). Hi-D: Nanoscale mapping of nuclear dynamics in single living cells. Genome Biology, 21(1). https://doi.org/10.1186/s13059-020-02002-6

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