Hi-D: Nanoscale mapping of nuclear dynamics in single living cells

25Citations
Citations of this article
92Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Bulk chromatin motion has not been analyzed at high resolution. We present Hi-D, a method to quantitatively map dynamics of chromatin and abundant nuclear proteins for every pixel simultaneously over the entire nucleus from fluorescence image series. Hi-D combines reconstruction of chromatin motion and classification of local diffusion processes by Bayesian inference. We show that DNA dynamics in the nuclear interior are spatially partitioned into 0.3-3-μm domains in a mosaic-like pattern, uncoupled from chromatin compaction. This pattern was remodeled in response to transcriptional activity. Hi-D can be applied to any dense and bulk structures opening new perspectives towards understanding motion of nuclear molecules.

Cite

CITATION STYLE

APA

Shaban, H. A., Barth, R., Recoules, L., & Bystricky, K. (2020). Hi-D: Nanoscale mapping of nuclear dynamics in single living cells. Genome Biology, 21(1). https://doi.org/10.1186/s13059-020-02002-6

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free