The mouthparts of the adult dragonfly Anax imperator (Insecta: Odonata), functional morphology and feeding kinematics

Abstract

Insects evolved differently specialized mouthparts. We study the mouthparts of adult Anax imperator, one of the largest odonates found in Central Europe. Like all adult dragonflies, A. imperator possesses carnivorous-type of biting-chewing mouthparts. To gain insights into the feeding process, behavior and kinematics, living specimens were filmed during feeding using synchronized high-speed videography. Additionally, the maximum angles of movement were measured using a measuring microscope and combined with data from micro-computed tomography. The resulting visualizations of the 3D-geometry of each mouthpart were used to study their anatomy and complement the existing descriptive knowledge of muscles in A. imperator to date. Furthermore, confocal laser scanning microscopy-projections allow for estimation of differences in the material composition of the mouthparts' cuticle. By combining all methods, we analyze possible functions and underlying biomechanics of each mouthpart. We also analyzed the concerted movements of the mouthparts; unique behavior of the mouthparts during feeding is active participation by the labrum and distinct movement by the maxillary laciniae. We aim to elucidate the complex movements of the mouthparts and their functioning by combining detailed information on (1) in vivo movement behavior (supplemented with physiological angle approximations), (2) movement ability provided by morphology (morphological movement angles), (3) 3D-anatomy, and (4) cuticle composition estimates.