Device for obtaining three-dimensional cell cultures, method for the implementation thereof, and use of such device.

Abstract

A device for obtaining three-dimensional cell cultures comprises: a substrate (1); and a plurality of micro-structures (2) which protrude from the surface of the substrate (1) and are arranged on such substrate (1) according to a periodical grid so as to make the substrate (1) super hydrophobic. The device is characterized in that the micro-structures (2) have side walls at least partially nano-patterned and have projections (14) and recesses (16) alternated with a predetd. distance. Each micro-structure (2) comprises a silicon core, a biocompatible coating, and possibly an adhesion layer for said biocompatible coating, applied to the surface of the substrate. A method for producing the device is also presented. [on SciFinder(R)]