Elevated levels of DNA strand breaks induced by a base analog in the human cell line with the P32T ITPA variant

Abstract

A encodes for the enzyme with a P32T amino-acid change and leads to accumulation of nonhydrolyzed ITP. The polymorphism has been associated with adverse reaction to purine base-analog drugs. The level of both spontaneous and HAP-induced DNA breaks is elevated in the cell line with the ITPA P32T variant. The results suggested that human ITPA plays a pivotal role in the protection of DNA from noncanonical purine base analogs. © 2010 Irina S.-R. Waisertreiger et al.