Tips for preparing mRNA-Seq libraries from poly(A)+ mRNA for illumina transcriptome high-throughput sequencing

Abstract

Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing. © 2013 Cold Spring Harbor Laboratory Press.