Evidence for the association of the human regulatory protein Ki-1/57 with the translational machinery

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Abstract

Ki-1/57 is a cytoplasmic and nuclear protein of 57 kDa first identified in malignant cells from Hodgkin's lymphoma. Based on yeast-two hybrid protein interaction we found out that Ki-1/57 interacts with adaptor protein RACK1 (receptor of activated kinase 1), CIRP (cold-inducible RNA-binding protein), RPL38 (ribosomal protein L38) and FXR1 (fragile X mental retardation-related protein 1). Since these proteins are involved in the regulation of translation we suspected that Ki-1/57 may have a role in it. We show by immunoprecipitation the association of Ki-1/57 with FMRP. Confocal microscopy revealed that Ki-1/57 colocalizes with FMRP/FXR1/2 to stress granules. Furthermore Ki-1/57 cosediments with free ribosomal particles and enhances translation, when tethered to a reporter mRNA, suggesting that Ki-1/57 may be involved in translational regulation. Structured summary of protein interactions:: Ki-1/57 and TIA-1 colocalize by fluorescence microscopy (View interaction) Ki-1/57 physically interacts with CIRP by two hybrid (View interaction) FMRP physically interacts with Ki-1/57 by anti bait coimmunoprecipitation (View interaction) Ki-1/57 physically interacts with FXR1 by two hybrid (View interaction) Ki-1/57 physically interacts with RPL38 by two hybrid (View interaction) © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Gonalves, K. D. A., Bressan, G. C., Saito, Â., Morello, L. G., Zanchin, N. I. T., & Kobarg, J. (2011). Evidence for the association of the human regulatory protein Ki-1/57 with the translational machinery. FEBS Letters, 585(16), 2556–2560. https://doi.org/10.1016/j.febslet.2011.07.010

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