Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics

19Citations
Citations of this article
54Readers
Mendeley users who have this article in their library.

Abstract

Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein Mast/Orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that Mast accumulates at kinetochores early in mitosis, which is followed by a poleward streaming upon microtubule attachment. This leads to a reduction of Mast levels at kinetochores during metaphase and anaphase that depends largely on the microtubule minus end-directed motor cytoplasmic dynein. Surprisingly, we also found that co-depletion of Dynein rescues spindle bipolarity in Mast-depleted cells, while restoring normal microtubule poleward flux. Our results suggest that Mast and Dynein have antagonistic roles in the local regulation of microtubule plus-end dynamics at kinetochores, which are important for the maintenance of spindle bipolarity and normal spindle length.

References Powered by Scopus

Dynamic instability of microtubule growth

2550Citations
N/AReaders
Get full text

Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence

521Citations
N/AReaders
Get full text

Retrograde transport by the microtubule-associated protein MAP 1C

473Citations
N/AReaders
Get full text

Cited by Powered by Scopus

The tumor suppressor CDKN3 controls mitosis

73Citations
N/AReaders
Get full text

Cell and molecular biology of microtubule plus end tracking proteins. End binding proteins and their partners

56Citations
N/AReaders
Get full text

Function and regulation of dynein in mitotic chromosome segregation

53Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Reis, R., Feijão, T., Gouveia, S., Pereira, A. J., Matos, I., Sampaio, P., … Sunkel, C. E. (2009). Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics. Journal of Cell Science, 122(14), 2543–2553. https://doi.org/10.1242/jcs.044818

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 21

48%

Researcher 19

43%

Professor / Associate Prof. 4

9%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 23

50%

Biochemistry, Genetics and Molecular Bi... 19

41%

Physics and Astronomy 3

7%

Computer Science 1

2%

Save time finding and organizing research with Mendeley

Sign up for free