Sem-sterile culture of Gracilaria salicornia seaweed (parental) on various media

Abstract

Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.