A rapid chromosome-mapping method for cloned fragments of yeast DNA

Abstract

A rapid and generally applicable method is described for mapping a cloned yeast DNA segment to the chromosome(s) from which it originated. The method is based upon the recent finding that the integration into a yeast chromosome of a segment of the 2μ plasmid DNA results, in heterozygous diploids, in the specific loss of genetic information from the chromosome into which the 2μ DNA was integrated. After verification of the accuracy of the method using several genes whose position was known in advance, the method was used to locate the yeast actin gene, which lies on the left arm or chromosome VI, about 50 cM distal to CDC4.