Application of intermittent microwave irradiation to western blot analysis

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Abstract

We established a shortened protocol for Western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.

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Liu, Y. T., & Toyokuni, S. (2015). Application of intermittent microwave irradiation to western blot analysis. In Methods in Molecular Biology (Vol. 1314, pp. 185–1902). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2718-0_20

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