Application of Optical Fluorescence Spectroscopy for Studying Bee Abundance in Tropaeolum majus L. (Tropaeolaceae)

Abstract

Tropaeolum majus L. species produce flowers with all sorts of colors, from yellow to red. This work aimed to apply optical fluorescence spectroscopy to study bee abundance in T. majus, answering the following questions: (1) do corolla temperature and weather conditions affect the abundance of visiting bee species? (2) do flower color and corolla fluorescence affect the abundance of visiting bee species? (3) do red flowers attract more visiting bees? (4) is there a relationship between bee visits and flower compounds? The bee species Apis mellifera, Paratrigona lineata, and Trigona spinipes were the most observed in T. majus flowers. The latter was more active in the morning and preferred orange and yellow flowers. These colors also had higher temperatures and fluorescence emissions than did the red ones and those with yellow-red and orange-red nectar guides. Orange flowers emitted a broadband UV spectrum (between 475 and 800 nm). This range might be due to compounds such as hydroxycinnamic acid, flavonols, isoflavonoids, flavones, phenolic acid, and chlorophyll. Extracts from different T. majus corolla colors showed that flowers emit specific fluorescent signals, mainly related to bee color vision and learning, thus acting as a means of communication between bees and flowers. In this way, this information evidences the interaction between bees and T. majus flowers, allowing conservation actions for pollinators.