The correlation between total protein content and antioxidant activity of collagen isolated from a marine sponge Stylissa flabelliformis collected from North Lombok Indonesia coast

Abstract

Abstract.Collagen is a fibrous protein that has recently gained high attention from the pharmaceutical industry due to its benefits on the skin. Collagen can be isolated from various resources including marine sponges. Marine sponges are found in a large amount in Indonesia and has not been widely explored for its pharmacology benefits. Here we isolate collagen from a marine sponge Stylissa flabelliformis found in North Lombok Indonesia coast. The isolation of collagen was performed followed by total protein content analysis using modified Bradford protein assay and antioxidant activity measurement using 2,2-diphenyl-1-picrylhydrazyl(DPPH) assay. The total yield of the collagen isolate obtained was 3.5% and it had a total of 0.755mg/ml protein. DPPH assay has shown that the collagen isolate had antioxidant activity with an IC50 of 61.5 2.132 ppm. Based on Spearman correlation assay, the antioxidant activity was found to be correlated with the protein content of the isolate (r value=0.8). These results show the potency of using the collagen isolated from marine sponge Stylissa flabelliformis for further antioxidant benefits.