Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I

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Abstract

Crystals of site-specific DNA nickase Nb.BspDoI (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume. © 2005 International Union of Crystallography. All rights reserved.

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Kachalova, G. S., Rogulin, E. A., Artyukh, R. I., Perevyazova, T. A., Zheleznaya, L. A., Matvienko, N. I., & Bartunik, H. D. (2005). Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(3), 332–334. https://doi.org/10.1107/S174430910500309X

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