Optimization of Invertase Production Using Saccharomyces Cerevisiae MK under Varying Cultural Conditions

Abstract

A Saccharomyces cerevisiae MK was isolated from toddy sample. The effect of different production parameters such as pH, temperature, incubation time, carbon source, nitrogen source (organic and inorganic), inoculum concentrations, sucrose concentrations, metal ions, surfactants, amino acids, buffers, agricultural residues and agricultural residue concentration on invertase production by the isolated Saccharomyces cerevisiae MK strain were studied. The enzyme production was assayed in submerged fermentation (SmF). Maximum invertase activity was found at pH 6, 30°C, 48 hours, sucrose, yeast extract (organic nitrogen), ammonium chloride (inorganic nitrogen), 2% inoculum concentration, 2% sucrose concentration, calcium chloride, poly ethylene glycol, methionine, citrate buffer, orange peel-4%. A higher titre of invertase enzyme activity (0.48 ± 0.011 IU/ml) was obtained in the optimized production medium.