Reference ranges of lymphocyte subsets of healthy adults in Turkey

Abstract

Abstract: The aim of this study waste determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey. Materials and Methods: Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 ± 16.69). Results: The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 ± 8.44%, 1,680 ± 528 cells/μl; CD4: 47.37 ± 9.10%, 1,095 ± 391 cells/μl; CD8: 28.99 ± 5.99%, 669 ± 239 cells/μl; CD19: 10.96 ± 4.44%, 254 ± 122 cells/μl and CD56: 7.03 ± 3.26%, 161 ± 92 cells/μl, respectively. The ratio of CD4/CD8 was 1.68 ± 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05). Conclusion: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey. Copyright © 2005 S. Karger AG.