Analisis Senyawa Acetaminophen pada Spesimen Rambut Manusia Menggunakan Metode Kromatografi Gas-Spektrometri Massa (Gc-Ms)

Abstract

Paracetamol is a drug containing non opiod analgesics and non-steroidal antipyretics (AINS). This journal review aims to analyze and find out the content of paracetamol (acetaminophen) can be detected in biological samples of the hair of a patient undergoing paracetamol treatment therapy using the gas chromatography-Mass spectrometry (GC-MS) method and knowing how much influence hair length has on paracetamol (acetaminophen) concentrations.  Biological samples of human hair were obtained by two different methods, from volunteers and 10 patients taking paracetamol. Paracetamol in human hair is extracted using methanol, then the extraction results are privatized with BSTFA content mixed with 1% TMCS and the last stage is analyzed using the gas chromatography method-Mass spectrometry (GC-MS). The results showed that patients who had taken paracetamol showed that in their hair samples detected positive acetaminophen in the form of acetaminophen-TMS. The acetaminophen-TMS form is produced as a result of derivatization with BSTFA containing 1% TMCS. In the hair sample with a length of 0-3 cm obtained concentrations of 0.1761-0.3392 ng / mg hair samples, in hair samples with a length of 0-6 cm obtained concentrations of 0.2081-0.4845 ng / mg hair samples and in hair samples with a length of 0-10 cm obtained a concentration of 0.2473-0.5782 ng / mg hair samples. From this journal review shows acetaminophen compounds can be detected in human hair specimens in individuals undergoing acetaminophen therapy, then in derivatization treatment can optimize the results of acetaminophen analysis in hair specimens and on the length of human hair can affect the concentration of acetaminophen compounds with the GC-MS method.