Genome-wide analysis for determining RNA turnover is an advanced method in RNA biology that examines the specific half-life of nuclear noncoding RNA (ncRNA). In particular, a pulse-labeling method using uridine analogs enables the determination of RNA stability under physiologically undisturbed conditions. The technique involves pulse labeling of endogenous RNAs in mammalian cells with 5′-bromo-uridine (BrU), followed by measuring the chronological decrease of BrU-labeled RNAs using deep sequencing. The method is called BrU immunoprecipitation chase assay (BRIC) or BRIC through deep sequencing (BRIC-seq). Here, we describe a detailed protocol and technical tips for BRIC-seq.
CITATION STYLE
Tani, H., Imamachi, N., Mizutani, R., Imamura, K., Kwon, Y., Miyazaki, S., … Akimitsu, N. (2015). Genome-wide analysis of long noncoding RNA turnover. Methods in Molecular Biology, 1262, 305–320. https://doi.org/10.1007/978-1-4939-2253-6_19
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