Fluorescent ion measurement

Abstract

It is likely that cell behavior is regulated by the summation of subcellular transduction events and these are generally linked, nonlinearly, to subsequent stages of signal transduction. Hence, a full understanding of signal transduction can only arise from the analysis of subcellular behavior and this is one reason why microscopic imaging has become so important. To carry this idea further, since the cell is a three-dimensional (3D) structure, methods that provide 3D imaging (as in confocal and multi-photon methods) are likely to provide better insight than even widefield/deconvolution imaging which is, itself, an improvement on either widefield or simple photometric methods. © 2006, 1995, 1989 Springer Science+Business Media, LLC. All rights reserved.