A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer

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Abstract

A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies.

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Lou, U. K., Wong, C. H., & Chen, Y. (2020). A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer. RSC Advances, 10(14), 8087–8092. https://doi.org/10.1039/c9ra07858d

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