Cloncurry buffel grass mitigated Cr(III) and Cr(VI) toxicity in tomato plant

Abstract

Contamination of agricultural soil with chromium (Cr) ions has threatened global crop, human and ecosystem health. Its two oxidation states viz. Cr(III) and Cr(VI) are most stable and readily available to the plants. The study explored the impact of increasing exposure (up to 500 ppm) of Cr(III) and Cr(VI) on bio-physical traits of 15-day-old seedlings (in vitro) as well as 60-day-old tomato plant (in vivo), and highlighted the importance of buffel grass (Cenchrus pennisetiformis) in mitigating Cr levels in the tomato plants. In vitro, Petri plate bioassays with 13 different concentrations (20–500 ppm) of Cr(III) and Cr(VI) depicted the highly toxic effect of metal ions ≥ 200 ppm on all bio-physical traits of tomato seedlings. In vivo, soil spiked with Cr(III) and Cr(VI) (200, 300, and 400 mg/kg) was amended with 1% and 2% dry biomass of buffel grass. Phytotoxicity was higher in Cr(VI)-spiked soil compared with Cr(III)-spiked soil. Cr was mainly accumulated in tomato roots, and more Cr was translocated from roots to shoots from Cr(VI)-spiked soil than Cr(III)-spiked soil. Soil amendments with 2% weed biomass reduced metal toxicity in plants, particularly at 200 and 300 mg/kg of Cr. Protein profiles through SDS-PAGE revealed 12–50 kDa (mainly PR proteins) as an important region in tomato leaf, where many new bands were expressed under different treatments, particularly in the treatments provided with buffel grass. PCA-based biplot clearly separated Cr tolerance treatments from highly sensitive treatments. For the cultivation of tomato plants in Cr(III) and Cr(VI) contaminated soil (200 and 300 mg/kg), the biomass of Cloncurry buffel grass should be considered an effective and easily available phyto-management option.