The solution structure of the pH‐induced monomer of dynein light‐chain LC8 from Drosophila

Abstract

The structure of Drosophila LC8 pH‐induced monomer has been determined by NMR spectroscopy using the program AutoStructure. The structure at pH 3 and 30°C is similar to the individual subunits of mammalian LC8 dimer with the exception that a β strand, which crosses between monomers to form an intersubunit β‐sheet in the dimer, is a flexible loop with turnlike conformations in the monomer. Increased flexibility in the interface region relative to the rest of the protein is confirmed by dynamic measurements based on 15 N relaxation. Comparison of the monomer and dimer structures indicates that LC8 is not a domain swapped dimer.