Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation

Abstract

The effect of UV irradiation on the expression of the vaccinia virus genome was investigated in a cell-free system coupling transcription with translation. Exposure of vaccinia virus to an increasing dose of irradiation resulted in differential reduction in the syntheses of virus-specified polypeptides in the coupled system, with sensitivity being proportional to the size of the gene pro duct. This suggests that each translationally functional mRNA species produced in vitro by vaccinia virus cores is synthesized from an individual promoter site.