The unexplored roles of probiotic bacteria: In vitro anti-inflammatory and anthelmintic activity of Enterococcus Faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586

Abstract

Objective: The aim of the present study was to evaluate the in vitro anti-inflammation activity and anthelmintic potential of two novel isolated probiotic strains through Enterococcus faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586. Methods: In vitro anti-inflammatory activity was evaluated using protein denaturation inhibition method. Pheretima posthuma was used as a suitable in vitro model, and time of paralysis and death were used as parameters to evaluate anthelmintic potential of probiotic strains. Results: Lyophilized solutions of L. casei GM10 showed significant protein denaturation inhibition (56.20±0.86%) followed by E. faecium BM10 (52.28±0.31%) comparable to diclofenac (93.62±1.39%) at the maximum concentration of 250 μg/ml. Intracellular cell-free extract of E. faecium BM10 showed a strong anthelmintic (vermicidal) activity (6±0.23 minutes), followed by L. casei GM10 (9±0.05 minutes) comparable to piperazine citrate (20±0.422 minutes) and albendazole (24±0.43 minutes) at the maximum concentration of 100 mg/ml. Conclusion: Results of the present study concluded that both tested lactic acid bacteria strains exhibited significant in vitro anti-inflammatory activity and can be used as potent and safe anthelmintic agent.