Studies on the interaction between zinc-hydroxybenzoite complex and genomic DNA

Abstract

Zinc-Hydroxybenzoite ([Zn (H 20) 6] (p-HO-C 6H 4COO) 22H 20) complex which was synthesized and characterized by instrumental methods and the DNA samples which had been isolated from cattle were allowed to interact at 37 °C for different time periods. The interaction of genomic DNA with this complex has been followed by agarose gel electrophoresis at 50 V for 2 h. When DNA samples were allowed to interact with this metal complex, it was found that band intensities changed with the concentrations of the complex. In the result of interaction between this complex and genomic DNA samples, it was determined that the intensities of bands were changed at the different concentrations of the complex. The brightness of the bands was increased and mobility of the bands was decreased, indicating the occurrence of increased covalent binding of the metal complex with DNA. In this study it was concluded that the damage effect of ascorbate was reduced by Zinc-Hydroxybenzoite. © 2006 by MDPI.